Chapter 3 Individual Operations
3.16 Fluorescence Observation
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3
3.16.7
ND filters for fluorescence attachment
Push in the ND filter attach/detach lever to move
ND filters into the optical path and darken the
fluorescent image.
ND filters are used to adjust light intensity. Higher
filter numbers correspond to lower transmission
rates (i.e., darker images). ND filters do not affect
color balance.
ND2: Reduces light intensity by 1/2.
ND4: Reduces light intensity to 1/8 previous levels.
ND16: Reduces light intensity to 1/16 previous levels.
As shown below, you can combine these three
filters to achieve various levels of brightness.
Brightness ND4 ND8 ND16
1 - - -
1/4
○
- -
1/8 -
○
-
1/16 - -
○
1/32
○
○
-
1/64
○
-
○
1/128 -
○
○
1/512
○
○
○
(-: Outside optical path, ○: In optical path)
ND filter attach/detach lever