Nikon Eclipse 50i - Chapter 7 Cleaning and Maintenance; Lens and Filter Cleaning

74
Troubleshooting
6
If the microscope does not function property, take appropriate action as described below. If the
problem is still not resolved after referring to "Troubleshooting," please contact your nearest
Nikon representative
6.1
Optical
Problem Possible causes Remedy
Parts installed incorrectly
Install the Parts (nosepiece, condenser,
etc.) correctly.
Movable parts not switched correctly
Rotate the parts (e.g., optical path
switchover dial, nosepiece , filter cube
switchover dial) until you feel resistance.
Field diaphragm image not focused
on the specimen surface
Focus and center the condenser.
Field diaphragm stopped down too
far
Open the field diaphragm slightly wider
than the field of view.
Field of view vignetting
Uneven illumination
across the field of view
Field of view not visible
Dirt or dust on lens and container Clean. Use a clean container.
Dirt or dust on lens and container Clean. Use a clean container.
Dirt or dust in the field
of view
Field diaphragm image not focused
on the specimen surface
Focus and center the condenser.
Dirt or dust on lens and container Clean. Use a clean container.
Objective's correction ring not
matched to the thickness of the
bottom plate container.
Adjust the ring correctly.
Poor image quality
Poor contrast
Poor resolution
Field diaphragm image not focused
on the specimen surface
Focus and center the condenser.
Uneven focus
Revolving nosepiece not installed
correctly, or not rotated to the click
stop position
Install correctly and rotate to the click
stop position.
Specimen tilted relative to stage
surface.
Correctly reposition specimen on stage.
Image is in motion.
Revolving nosepiece not installed
correctly, or not rotated to the click
stop position
Install correctly and rotate to the click
stop position.
Yellow-tinged Lamp voltage too low (for 50i)
Adjust the brightness adjustment dial to
match the lamp ratings.
No ND filter in optical path Place filter in optical path.
Field of view too bright
Lamp voltage too high (for 50i)
Reduce the voltage with the brightness
adjustment dial.
Condenser aperture diaphragm
stopped down too far
Should normally be set to 70 to 80% of
the objective N.A.
Field diaphragm image not focused
on the specimen surface
Focus and center the condenser.
Field of view too dark
Optical path switchover dial not set
to 100% eyepiece
Switch to 100% eyepiece.