19
II. Microscopy
5
Pull out the polarizer slider on the epi
illuminator to remove the polarizer from
the optical path.
See Page 36.
6
Push in the analyzer slider on the
polarizing intermediate tube to remove the
analyzer from the optical path.
See Page 37.
7
Move the Bertrand lens turret to the “0”
position to remove the Bertrand lens from
the optical path.
See Page 39.
8
Push in the illumination selector lever on
the epi illuminator to set the bright-field
(BF) position for it.
See Page 42.
9
Rotate the nosepiece to place the 10x
objective into the optical path.
10
Set the specimen in place with the cover
glass facing up.
11
Fully open the field diaphragm and the
aperture diaphragm on the epi illuminator.
See Pages 43 and 44.
12
When using the trinocular eyepiece tube,
set the optical path selector lever to the
position where 100% light goes through
the binocular eyepiece.
See Page 27.
13
Adjust the diopters and the interpupillary
distance.
See Page 28.
14
Focus on the specimen.
Fully loosen the coarse focus stopper ring.
See Page 25.
15
Center the objective.
See Page 33.
16
Center the field diaphragm.
See Page 43.
N
D
8
NC
B
F.S
.
0
.4
0
.
2
0
.
8
0.
6
J
A
P
A
N
A
chr
0
.
9
0
F
.
STOP
A
.
STOP
BF
D
F
0
BINO
PHOTO
PHO
T
O
BINO
&
6
Remove the analyzer
from the optical path.
7
Move the
Bertrand
lens to the
“0” position.
8
Set to the “BF”
(bright-field)
position.
5
Remove the
polarizer from
the optical path.
F
.
STOP
A
.
STOP
BF
DF
9
Set the 10x
objective.
11
Fully open the field
diaphragm and the
aperture diaphragm.
10
Set the
specimen.
Cover glass
facing up
F
.
STOP
A
.
STOP
0
BINO
PHO
T
O
P
HOT
O
BINO &
12
Set to the BINO position.
13
Adjust the interpupillary distance.
13
Adjust the diopters.
ND8
NCB
F.S .
0
.4
0
.
2
0
.
8
0.
6
J
A
P
A
N
A
c
hr
0
.
9
0
F
.
STOP
A
.
STOP
BF
D
F
0
BINO
PHOTO
PHO
T
O
BINO
&
14
Loosen the coarse focus
stopper ring and focus on
the specimen.
15
Center the
objective.
16
Center the
field
diaphragm
(both sides).
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