33
III. Operation of Each Part
14
Centering the Objective
To perform the polarization microscopy, the center of the objective optical path must be aligned to
the rotation center of the circular graduated stage. This product comes with the centering nosepiece.
You can perform the centering adjustment for each objective.
Required tools two centering tools (provided with the nosepiece)
1 Before centering the objectives, focus on a
specimen using the 10x objective.
2 Bring an appropriate target such as granules
that can be easily used as a marker in the
specimen to the center of the crosshairs of
the eyepiece.
3 Insert the centering tools into the centering
screws on the nosepiece.
4 Rotate the stage about 180 degree. Move
the objective using the centering tools so
that the center of the crosshairs moves by
one-half the amount of movement of the
target.
5 Move the specimen and bring the target to
the center of the crosshairs.
6 Repeat this procedure several times. Carry
out this centering procedure for each
objective.
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Objective
centering tools
Middle point
of the
movement
Target
Rotate 180°
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