21
II. Microscopy
5
Photomicroscopy
For detailed information for the camera, photomicroscopic software, and PC, refer to the operating manuals
provided with the respective products. The following instructions assume that the digital camera DS-5M and
the camera control unit DS-L1 are used.
1
Adjust the microscope for proper image observation.
Refer to Page 12, “1 Diascopic Bright-field Microscopy” through Page 18, “4 Episcopic Microscopy.”
2
Adjust the camera head attaching position until the image is displayed properly on the
monitor.
Loosen the attachment guide fixing screw on the C mount and adjust the camera position so that moving
the stage left-right moves the image on the monitor in the opposite direction. After making the
appropriate adjustments, tighten the screws firmly.
3
Make camera settings.
For detailed information, refer to the instruction manual provided with the camera.
When using the DS-L1, you must choose and enter at least the following information:
• Folder for data storage
• Name of the file to be saved (You can select “Auto.”)
• File format and file size
• Date and destination of data
4
Select the camera scene mode suitable for the microscopy method.
5
Set the camera white balance.
To adjust the white balance, press the WB button while capturing an image of a clear section of a
specimen slide.
For fluorescent photomicrography, adjust the white balance under normal lighting conditions before the
image capturing.)
6
Capture and save images.
• Move the stage to get the target on the specimen.
• Readjust the focus.
• Adjust the image brightness using the camera exposure compensation function.
• Check the image using the Freeze button function.
• When the image is acceptable, press the CAPT. button to save the image.
(The operating procedure differs if DF/FL scene mode is selected. For details, see the manual for the
camera.)