Chapter 2 Microscopy
2.4 External Phase Contrast Microscopy
47
5
Change the objective.
Coarse
Fine
ExFine
Obj.
ON
OFF
6V30W
MAX
.
12V100W
L80
EYE
DISPLA
Y
MEMORY
PFS
ON
RECALL
Z
-
RESET
BRIGHTNESS
R100
L100
FOCUS
1. Move another external phase contrast
objective into the optical path by pressing the
Obj. switch on the left operation panel.
2. Rotate the eyepiece base unit turret to the
position for the external phase contrast ring
that is suitable for the objective in the optical
path.
3. Rotate the condenser turret to the position
for the Ph code of the objective.
4. Center the annular diaphragm of the
condenser.
For the centering procedure, refer to step 3 on
page 46.
6
Change the specimen.
B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS
OFFSET
OUT
DICHRO
IC
M
IR
ROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z
-
RESET
1X
1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
S
I
D
E
E
Y
E
Use the following functions as necessary.
• Inclining of dia pillar illuminator
When using the 100W dia pillar illuminator, the
entire dia pillar illuminator can be inclined
backward by loosening the fixing knob on its
back side, so as to secure more working space.
• Escape switch, Refocus switch
If there is a need to lower the objective, the
objective can be retracted temporarily by
pressing the Escape switch on the right
operation panel.
To return the objective to approximately the
same height, press the Refocus switch without
rotating the focus knobs.
• Condenser refocusing clamp
If there is a need to move the condenser up and
down, tighten the condenser refocusing clamp
before moving the condenser. This will make it
easier to restore the condenser to the original
position.
5-1
5-3, 5-4
5-2
Condense
refocusing clamp
Escape switch,
Refocus switch
Inclining of
dia pillar
illuminator