Chapter 4 Assembly
92
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Attaching the eyepiece base unit
(1) Attaching TI-T-B, TI-T-BS
Figure 4-5 Eyepiece Base Unit attachment
(TI-T-B, TI-T-BS)
1. Place the eyepiece base unit onto the front
part of the microscope base, so that the
eyepiece tube mount of the eyepiece base
unit faces the front.
There are two positioning pins on the bottom of
the eyepiece base unit. Align these pins with the
holes on the microscope base.
2. Secure the eyepiece base unit by tightening
four hex socket head screws with the
provided 4 mm hex screwdriver.
(2) Attaching the TI-T-BPH External Phase Contrast Eyepiece Base Unit
To perform external phase contrast microscopy, attach an external phase contrast eyepiece base unit onto
the microscope base, and attach a phase plate suitable for the selected condenser lens and objective to the
eyepiece base unit.
Figure 4-6 External phase contrast eyepiece base
unit attachment
1. Place the TI-T-BPH External Phase Contrast
Eyepiece Base Unit onto the front part of the
microscope base.
There are two positioning pins on the bottom of
the eyepiece base unit. Align these pins with the
holes on the microscope base.
2. Secure the eyepiece base unit by tightening
four hex socket head screws with the
provided 4 mm hex screwdriver.
Selecting objectives and phase plates
The phase plate must be selected to suit the objective being used. Refer to the following table to select an
objective and an appropriate phase plate. Up to three phase contrast plates (A, B, and C) can be attached to
an external phase contrast eyepiece base unit.
Condenser phase contrast code
(Supported condenser lens)
Objective
External phase
contrast ring
1 Ph3 (LWD, CLWD) P Apo 60x WI (NA1.2) 60x/Ph3
2 Ph3 (LWD, CLWD) P Apo VC 60x WI (NA1.2) 60x/Ph3
3 Ph3 (LWD, CLWD) P Apo VC 60x H (NA1.4) 60x/Ph3
4 Ph4 (CLWD) Apo TIRF 60x H (NA1.49) 60x/Ph4
5 Ph4 (CLWD) P Apo TIRF 60x H (NA1.45) 60x/Ph4
6 Ph3 (LWD, CLWD) P Apo VC 100x H (NA1.4) 100x/Ph3
7 Ph4 (CLWD) Apo TIRF 100x H (NA1.49) 100x/Ph4
Eyepiece base unit
Positioning holes (x2)
External phase contrast
eyepiece base unit
Positioning holes (x2)
Hexagonal socket head bolts
(Built into the eyepiece
base unit) (M5, x4)
Hexagonal socket head bolts
(Built into the eyepiece
base unit) (M5, x4)
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