PacBio Onso - Required Materials and Equipment

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PN 103-140-400 REV05 JUN2024
Required materials and equipment
Clustering Materials
Supplier/Manufacturer
Onso™ clustering reagent kit
PacBio
®
102-855-900
Onso™ clustering reagent plate
PacBio
®
102-752-200
Library dilution buffer (DIL buffer)
PacBio
®
102-386-600
Onso™ flow cell kit
PacBio
®
103-064-700
Wash cartridge set
PacBio
®
102-932-300
Empty troughs
provided in accessory kit (PacBio
®
102-673-800)
Onso™ clustering instrument wash plate
PacBio
®
102-673-600
Onso™ cluster generator system (instrument)
PacBio
®
102-837-100
Nuclease-free water, molecular biology grade
Any Major Lab Supplier (MLS)
Sodium Hydroxide (NaOH), ≥ 1N
Any MLS
Low TE buffer (10 mM Tris, 0.1 mM EDTA, pH 8.0)
Any MLS
DNA low-bind tubes
Any MLS
Bleach (NaClO), ≥ 5%
Any MLS
Tween-20, molecular biology grade, prepared to a 10% (w/v)
Any MLS
100X TE Buffer, molecular biology grade
Any MLS
Vortexer
Any MLS
Microcentrifuge
Any MLS
Pipettes, 2-1000 µL pipetting range
Any MLS
Pipette tips, 2-1000 µL
Any MLS