Contents
Before you begin ......................................................................................................................................................... 5
Flow cell and Main Instrument Connector Assembly (MICA) configuration ..................................................................... 5
Flow cell configuration............................................................................................................................................................. 5
MICA configuration .................................................................................................................................................................. 6
a)
Connecting MICA instrument connection side to instruments ............................................................................... 6
b)
Disconnecting MICA instrument connection side from instruments ..................................................................... 6
Powering Instruments On ........................................................................................................................................................... 6
Safety guidelines ........................................................................................................................................................................... 7
General best practices ................................................................................................................................................ 7
DNA library input ........................................................................................................................................................................... 7
Multiplexing .................................................................................................................................................................................... 7
Library loading concentration and control spike-in guidelines ............................................................................................. 7
Reagent and sample handling .................................................................................................................................................... 8
Clustering on the cluster generator ............................................................................................................................. 8
Cluster generator .......................................................................................................................................................................... 9
Clustering workflow .................................................................................................................................................. 10
Required materials and equipment ........................................................................................................................... 11
Clustering procedure ................................................................................................................................................ 12
1. Thawing clustering reagents ............................................................................................................................................... 12
2. Preparing instrument wash reagent .................................................................................................................................. 12
3. Denaturing and diluting the DNA library ............................................................................................................................ 12
4. Onso clustering reagent plate preparation ....................................................................................................................... 15
5. Cluster generator setup and run ......................................................................................................................................... 16
6. Post-run wash ........................................................................................................................................................................ 23
7. Instrument washes ............................................................................................................................................................... 26
8. Long-term shutdown ............................................................................................................................................................ 28
Sequencing ............................................................................................................................................................... 29
Onso system short-read sequencer ....................................................................................................................................... 29
Sequencing workflow................................................................................................................................................ 30
Required materials and equipment ........................................................................................................................... 30
Sequencing procedure .............................................................................................................................................. 31
1. Thawing sequencing reagent pack thaw .......................................................................................................................... 31
2. Optional custom primer addition ........................................................................................................................................ 31
To Next Page