© 2024 PacBio. All rights reserved. For research use only. Not for use in diagnostic procedures.
PN 103-140-400 REV05 JUN2024
Troubleshooting Instructions
Sequencing instrument does not
connect to software
Check to ensure a wired connection exists between the instrument and PC. Power off
the sequencer and exit the sequencing software. Exiting the software will cause the PC
to shut down. Restart the sequencer, wait 30 seconds, then power on the sequencer.
If trouble persists, contact your PacBio field service and support.
Preload does not
engage/disengage
If the preload does not engage during pre-run checks, re-initialize the pre-run checks.
If the preload does not disengage after a run has completed, restart both the
instrument and PC.
If the problem persists, contact your PacBio field service and support.
Check the MICA or wash cartridge connection by pinching the grooves on the MICA or
wash cartridge and gently lifting up to release. Reseat the MICA or wash cartridges into
the instrument ports and retry the pre-run check.
If reconnecting the MICA does not resolve the issue, unload the flow cell and MICA and
perform a maintenance wash.
If the maintenance wash completes, retry loading the flow cell consumable.
If the maintenance wash fails, contact your PacBio field service and support.
Sequencing reagent pack not
detected
Ensure that the reagent pack is pushed all the way into the internal compartment of the
reagent door. The reagent door must be able to close without obstruction. If the
reagent pack does not fully fit into the reagent door internal compartment, remove the
sequencing reagent pack and check for any unexpected obstructions in the drawer.
If no obstruction is detected, re-insert the reagent pack, ensuring that the primers are
oriented towards the right-hand side, and the sequencing reagent pack barcode is
facing the user when inserted into the instrument. Gently push the reagent pack all the
way to into the internal compartment of the reagent door and close the reagent door.
See section titled “Run management” for instruction.
If trouble persists, contact your PacBio field service and support.
Revision history (description)
Updated screenshots referencing “workspaces” door.
Updated maintenance wash to 7 days instead of 14 days. Other minor
updates throughout.
Updated software screens, addition of standby wash, and other minor
updates throughout.
Added additional pinch hazard warnings for the cluster generator drawer
Research use only. Not for use in diagnostic procedures. © 2024 Pacific Biosciences of California, Inc. (“PacBio”). All rights reserved.
Information in this document is subject to change without notice. PacBio assumes no responsibility for any errors or omissions in this
document. Certain notices, terms, conditions and/or use restrictions may pertain to your use of PacBio products and/or third-party
products. Refer to the applicable PacBio terms and conditions of sale and to the applicable license terms at pacb.com/license. Pacific
Biosciences, the PacBio logo, PacBio, Circulomics, Omniome, SMRT, SMRTbell, Iso-Seq, Sequel, Nanobind, SBB, Revio, Onso, Apton,
Kinnex, and PureTarget are trademarks of PacBio.
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