PacBio Onso - Instrument Washes

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PN 103-140-400 REV05 JUN2024
Wash cartridges should stay connected to the instrument inside the workspace door until the next
run is loaded but can be retained at room temperature for up to 30 days after initial use.
7. Instrument washes
There are two types of instrument washes outside of the standard clustering workflow: 1) standby wash, and 2)
maintenance wash. A standby wash is required if the instrument is idle for ≥5 days and lasts approximately 30
minutes. A maintenance wash is required if the instrument is idle for ≥10 days and lasts approximately 60 minutes.
Both types of washes require the use of instrument wash reagents, which are to be prepared as described in the
section titled “Prepare instrument wash reagent” above. To perform an instrument wash, a clustering instrument
wash plate is required along with four empty troughs supplied in the accessory pack.
The cluster generator will require a standby wash in one or more of the following scenarios: 1) if pre-run checks fail
and 2) if the instrument is idle for ≥5 days.
The cluster generator will require a maintenance wash in one or more of the following scenarios: 1) used routinely
every 7 days, 2) if a run is cancelled, and 3) if the instrument is idle for ≥10 days thus preparing for long-term
shutdown.

Step
Instructions
7.1
Rinse the clustering instrument wash plate and four empty troughs with DI water prior to use and dry with a
lint-free towel.
7.2
Load the four empty troughs onto the plate and ensure they are pressed firmly down until the tip of each
trough meets the plate.
7.3
On the clustering instrument screen, click on the top right hamburger menu and select “Washes”.