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PN 103-140-400 REV05 JUN2024
6. Instrument washes
There are two types of instrument washes outside of the standard sequencing workflow: 1) standby wash, and 2)
maintenance wash. Both types of washes require the use of the sequencing instrument wash pack and contains
two wash steps requiring user interaction between them. The first wash step is performed with diluted bleach
(prepared as described below), and the second wash step is performed using instrument wash reagent (prepared
as described in section 2 of the Onso clustering section of this guide).
The Onso sequencing instrument will require a standby wash in one or more of the following scenarios: 1) if pre-run
checks fail and 2) if the instrument is idle for ≥5 days. For a standby wash, the first wash step using diluted bleach
lasts approximately 20 minutes, and the second wash step using instrument wash reagent lasts approximately 20
minutes.
The Onso sequencing instrument will require a maintenance wash in one or more of the following scenarios: 1)
used routinely every 7 days, 2) if a run is cancelled, and 3) if the instrument is idle for ≥10 days thus preparing for
long-term shutdown. For a maintenance wash, the first wash step using diluted bleach lasts approximately 20
minutes, and the second wash step using instrument wash reagent lasts approximately 2 hours.
Prepare 1 L of 0.1% NaClO (Bleach) solution, and 1 L of instrument wash reagent. See section 2 of Onso
clustering, titled “Prepare instrument wash reagent”.
Rinse the sequencer instrument wash pack with DI water prior to use. Dry the instrument wash pack by
inverting and tapping gently upside down and dry the exterior with a lint-free wipe.
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