PacBio Onso - Sequencing Workflow; Required Materials and Equipment

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PN 103-140-400 REV05 JUN2024
reagent pack are housed within the reagent door. The waste container should be emptied and returned to its
correct position within the instrument prior to starting a sequencing run, and during washes. Sequencing reagent
packs are single-use and contain a barcode which should be visible when the reagent pack is properly loaded on
the sequencing instrument.
Sequencing workflow
Required materials and equipment
Sequencing Materials
Supplier/Manufacturer
Onso™ sequencing system
PacBio
®
102-837-000
Clustered flow cell with attached MICA
Prepared by user
Onso™ sequencing reagent kit
200-cycle kit: PacBio
®
102-656-400
300-cycle kit: PacBio
®
102-700-700
Reagent pack removal tools
PacBio
®
provided in accessory kit (PacBio
®
102-512-502)
Onso™ sequencer instrument wash pack
PacBio
®
provided in accessory kit
Instrument wash reagent
Prepared by user (see section 2 of “Onso clustering”)
Bleach (NaClO), ≥5%
Any Major Lab Supplier (MLS)
70% Isopropyl Alcohol
Any MLS
Lint-free wipes
Kimtech or other MLS
Serological Pipettor
Any MLS
Serological Pipettes (10 mL, 25 mL, 50 mL, or 100 mL)
Any MLS
1 Sequencing reagent kit thaw
2 Optional custom primer addition
Sequencer setup and run
3 and
automatic post-run wash
16-24 hours (no water bath)
OR 2 hours (with water bath)
5 minutes
200-cycle: 32 hours
300-cycle: 48 hours