User Manual PEAK INSTRUMENTS INC
2
quantity of the dangerous material being used.
2. Product overview
2.1. Basic operating principles
2.1.1. The nature of absorption: spectrophotometric analysis method is the use
of substances to choose a different wavelength of light absorption
characteristics established. Typically using a prism or grating to obtain
monochromatic light that passes through the continuous monochromatic
solution, the solution was measured and the absorption of each wavelength, the
absorption spectrum curve obtained.
Absorption spectrum selective absorption of light from the material, which is the
material of macroscopic phenomena, and the nature of the molecular absorption
is the result of internal movement and light interaction. When molecules absorb
certain wavelengths of spectral energy or by some wavelengths of the spectrum
is absorbed to form the absorption spectra. The smaller the energy absorption,
the wavelength of light corresponding to the absorption peak at a longer
wavelength. When the infrared absorbent is formed in the infrared absorption
spectrum, if the energy absorption is larger, the shorter the wavelength
corresponding to the absorption peak at a shorter wavelength, when generating
the ultraviolet absorption spectra of absorption in the ultraviolet region.
2.1.2. Absorption Law - Lambert Beer law: when a parallel beam through the
homogeneous solution, the absorbance of monochromatic light is proportional to
the product of the solution concentration and thickness.
Its digital expression: A=KCL=LogI/I
0
=-LogT.
Premise absorption laws established numeric expression: ① the incident light is
monochromatic ② the absorption process without interaction of each substance,
the absorbance of each substance has additivity ③ the role of light and matter is
limited to the absorption process, no fluorescent and photochemical scattering
phenomena ④ absorbent system is a continuous uniform distribution
2.1.3. Impact spectrophotometry factors:
2.1.3.1. Non-absorption errors caused by radiation and matter
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