Lambda 20, Lambda 40 UV/Vis Spectrometers
54
Use and Care of Cells
Cell Handling
A good spectrometer cell is an optical device, forming a part of the optical system of
the instrument with which it is used. It must be accorded the same careful treatment
applied to any optical component. Optical faults of a minor nature, scratches, lint,
fingermarks, etc. on the optical surfaces can easily introduce substantial analytical
errors.
You should observe the following list of cell handling rules to prevent analytical
errors and to achieve utmost precision:
• Only hold cells by non-optical surfaces, such as the matt finish surfaces.
• Protect cells from scratches, and never permit them to rub against one another
or against other hard surfaces.
• Avoid abrasive, corrosive or stain-producing cleaning agents, and make certain
that the exposed surfaces of cells are optically clean.
• Always wipe the optical surfaces of cells dry and free of fingermarks, using a
soft cloth or cleaning tissue, just before placing them in the cell holder.
• When measuring cold solutions, always bear in mind that condensation can
form on the optical surfaces.
• Make certain no bubbles cling to the inner surfaces of the cell, particularly
when handling cold solutions.
• For maximum precision and accuracy, calibrate and test with cells of the same
type, and always insert cells into the holders with the same orientation.
Pressure Buildup in Cells
If you are using stoppered cells, observe the following rules to prevent the buildup
of internal pressure that could cause the cell to burst:
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