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Photon Systems Instruments FluorPen FP 110 - 7 Getting Started; Pulses Description and Setting

Photon Systems Instruments FluorPen FP 110
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© PSI (Photon Systems Instruments), spol. s r. o.
16
7 GETTING STARTED
For more detailed information on particular steps of FluorPen operation please refer to chapter 8.
The device can is powered with built in Li-Ion battery. Ensure the battery is fully charged by plugging it into a PC via USB
cable or the AC outlet via the USB cable and a USB adaptor (not included). Monitoring Pen can be powered from an
optional battery pack (see more in chapter 15.1)
The FluorPen is controlled using two buttons:
Use the MENU key to scroll through sequential menu options on the digital display and to turn the device off
(hold for 1s)
Use the SET key to turn the device on (hold for 1 sec) and select a menu option based on cursor (>) position.
7.1 PULSES DE S CRIPTION A N D SE TTING
Flash pulse
This function serves for setting of measuring pulses intensity. The measuring pulses are weak light pulses, which are able
to induce the minimal chlorophyll fluorescence (F
0
or F
t
). It takes only 30 µs and the maximum intensity is
3,000 µmol.m
-2
.s
-1
. It means 30 µs * 3,000 µmol.m
-2
.s
-1
= 0.09 µmol.m
-2
per pulse is the maximal intensity of the flash pulse.
Super pulse
This function serves for setting intensity of the saturating light pulse. Saturating pulse induces maximum chlorophyll
fluorescence (F
m
). 100 % of intensity equals approximately 3,000 µmol.m
-2
.s
-1
.
Actinic pulse
This function serves for setting intensity of measuring pulses. Actinic light is the ambient light in which the algae are
growing. 100 % of intensity equals approximately 1,000 µmol.m
-2
.s
-1
.
Pulses used in predefined protocols:
Measurements based on fluorescence
Used pulses
F
t
Flash pulse
QY
Flash pulse, Super pulse
OJIP
Super pulse
NPQ protocols
Flash pulse, Super pulse, Actinic pulse
Light Curves
Flash pulse, Super pulse (Actinic pulse is preset)
Default setting of light intensities in the FluorPen firmware. These may be changed according to user requirements
and algal growth conditions:
Flash pulse 30 % = Measuring flash pulse
Super pulse 80 % = Saturating pulse
Actinic pulse 300 µmol.m
-2
.s
-1
(30 %) = Actinic light
Please note that those paramets are recommended by the manufacturer but canbe changed by the user
according to requirements.