Photon Systems Instruments FluorPen FP 110 - Page 20

© PSI (Photon Systems Instruments), spol. s r. o.
20
The OJIP protocol includes the following measured and calculated parameters:
Abbreviation
Explanation
Bckg
Background
F
0
F
0
= F
50µs
, fluorescence intensity at 50 µs
F
j
F
j
= fluorescence intensity at J-step (at 2 ms)
F
i
F
i
= fluorescence intensity at i-step (at 30 ms)
F
m
F
m
= maximal fluorescence intensity
F
v
F
v
= F
m
- F
0
(maximal variable fluorescence)
V
j
V
j
= ( F
j
- F
0
) / ( F
m
- F
0
)
V
i
V
i
= ( F
i
- F
0
) / ( F
m
- F
0
)
F
m
/ F
0
F
V
/ F
0
F
v
/ F
m
M
0
or (dV/dt)
0
M
0
= TR
0
/ RC - ET
0
/ RC = 4 ( F
300
- F
0
) / ( F
m
- F
0
)
Area
Area between fluorescence curve and F
m
(background subtracted)
Fix Area
Area below the fluorescence curve between F
40µs
and F
1s
(background subtracted)
S
M
S
M
= Area / ( F
m
- F
0
) (multiple turn-over)
S
S
S
S
= the smallest S
M
turn-over (single turn-over)
N
N = S
M
. M
0
. ( 1 / V
J
) turn-over number Q
A
Phi_P
0
Phi_P
0
= 1 – ( F
0
/ F
m
) (or F
v
/ F
m
)
Psi_
0
Psi_
0
= 1 - V
J
Phi_E
0
Phi_E
0
= ( 1 – ( F
0
/ F
M
)) . Psi_
0
Phi_D
0
Phi_D
0
= 1 – Phi_P
0
= ( F
0
/ F
m
)
Phi_Pav
Phi_Pav = Phi_P
0
( S
M
/ t
Fm
) t
Fm
= time to reach F
m
(in ms)
ABS / RC
ABS / RC = M
0
. ( 1 / V
J
) . (1 / Phi_P
0
)
TR
0
/ RC
TR
0
/ RC = M
0
. ( 1 / V
J
)
ET
0
/ RC
ET
0
/ RC = M
0
. ( 1 / V
J
) . Psi_
0
DI
0
/ RC
DI
0
/ RC = ( ABS / RC ) – ( TR
0
/ RC )
Formulas Derived From:
R.J. Strasser, A. Srivastava and M. Tsimilli-Michael (2000): The fluorescence transient as a tool to characterize and screen
photosynthetic samples. In: Probing Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P.
Mohanty, eds.), Taylor and Francis, UK, Chapter 25, pp 445-483.