PicoQuant MultiHarp 150 - Page 16

PicoQuantGmbH MultiHarpSoftwareV.3.1.0.0
Theapproximatemono–exponentialfluorescencelifetimecanbeobtainedfromasimplecomparisonoftwo
pointsinthecurvewithcountsintheratioof1:
1
/
e
(e.g.100000:36788).Foraprecisemeasurementone
wouldperformanumericalexponentialfitwithIRFdeconvolution(typicallyimplementedasaniterativereconvo-
lution).ThiswouldresultinslightlyshorterlifetimessincetheIRFbroadensthedecay.Indeedonecanmeasure
lifetimessignificantlysmallerthantheIRFwiththismethod.Additionally,theresiduesofthefitcanthenbeused
toassessthequalityofthefitandtherebythereliabilityofthelifetimemeasurement.The“EasyTau2”software
packagefromPicoQuantprovidesthisfunctionality.
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