PicoQuant MultiHarp 150 - Page 15

PicoQuantGmbH MultiHarpSoftwareV.3.1.0.0
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ThefollowingfigureshowsTCSPChistograms obtained withthiskind of setup.Excitationsource was a
PDL800–B(PicoQuant)witha375nmlaserheadrunningat20MHzrepetitionrate.DetectorwasaPMAHy-
brid06fromPicoQuantwithinternalamplifier.Thecut-offfilterwasalongpassfor>430nm.Thenarrowerpeak
(bluecurve)representsthesystemIRF,heredominatedbylaseranddetector.Theothercurve(red)isthefluo-
rescencedecayfromasolutionofATTO425inwater,afluorescentdyewithfairlyshortfluorescencelifetime
(~3.7ns).Thecountratewasadjustedto<3%ofthelaserratetosafelypreventpile-up.Theplotinlogarithmic
scaleshowstheperfectmono-exponentialnatureofthedecaycurve,asonewouldexpect.Notethatthisisob-
tainedwithoutadeconvolutionoftheIRF.
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