PicoQuant MultiHarp 150 - Page 56

PicoQuantGmbH MultiHarpSoftwareV.3.1.0.0
ATRESmeasurementcanthenbestartedorcanceledasusualviathetoolbarbuttons
forstartandstop. Thedataacquisitionwillbeperformedlikeastandard“Integration
Mode”measurement,whiletheblocknumberisincrementedforeachnewwavelength.
AtthebeginningofeachTRESrun,theactivecurve(block)willbesetto0,orto1ifnoIRFiscollected.All
curvesthatwerepreviouslycollectedandnotsavedtoafilewillbeoverwrittenwithnewdata.DuringaTRES
run,thecurrentlycollecteddatawillalwaysbeshownasTrace0(darkblue),eventhoughtheblock(Trace)
numberisactuallyincrementedateachwavelengthstep.Thisistoovercomethelimitationofonly16display
curvesbeingavailable.Youcanchecktheblocknumberinthecontrolpaneltoseethecurvecurrentlybeing
collected.Manualentrywillbedisabledduringtherun.Also,youcanwatchthestatusbartoseethecurrent
wavelengthandsteppingactivity.NotethatforaTRESmeasurementthecontrolpanelsetting'StopOf'applies
asinIntegrationmode,while'Restart'ismeaninglessinTRESmode.
TRES Data Analysis and Visualization
HavingcollectedacompleteTRESdatasetyoucansaveittoaregular*.phufile.Youcanalsoinspectindi-
vidualcurvesviatheTraceMappingdialog.YoucanfurthermoreusethesoftwaretoolFluoPlotfromPicoQuant
tovisualizeandanalyzethedatainvarious2Dand3Drepresentationswithamultitudeofoptionsforcoloring,
scaling,andchangingviewaspectsin3D.ThefiguresbelowshowFluoPlotvisualizationsfromaTRESmea-
surementofmixedoxazinedyesinethanol.
FluoPlotisprovidedonyoursoftwareinstallationmedia.JustrunFluoPlotSetup.exefromthere.Ifyoureceived
yourMultiHarpsoftwarebydownloadoremailyoumayneedtorequestFluoPlotseparately.Notethatthepro-
gramrequiresamoderngraphicsprocessingunitwithsupportforOpenGLversion1.5orhigher.Speedyhan-
dlingoflargedatafilesrequiressufficientsystemmemory.Furtherdataanalysismayneedtobeperformedby
dedicatedsoftware,eithercustomprogramsorspecializedsolutionsavailablefromPicoQuant.
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