PicoQuant MultiHarp 150 - Page 55

PicoQuantGmbH MultiHarpSoftwareV.3.1.0.0
Ifthereisalargedifferencebetweenthecurrentmonochromatorpositionandthepositionentered,themove-
mentwilltakesometime.Ifyouwishtointerruptthisprocess,presstheStopbutton.Themonochromatorwill
immediatelyslowdownandthenstop.Thedecelerationisnecessarytopreservethecalibration.
Running TRES Measurements
TheTRESpageofthedialogcontainsthecontrolsusedtosetupanautomatedscanningofthemonochroma-
tor.Thestartandstopwavelengthsofthescancanbeenteredinthecorrespondingeditboxes,theD(delta)
boxdefinesthestepwidthofthescan.ThescanstartsalwaysexactlyattheStartvalue.IftheStopposition
cannotbereachedbyanintegermultipleofthestepwidth,thescanstopsatthelastpositionwithinthe
start/stoprange.Ifthestartpositionislargerthanthestopposition,thescanningdirectionwillbereversed.
Themaximumrangeandnumberofwavelengthstepsisdeterminedbythephysicalcharacteristicsofthe
monochromatoraswellasthemaximumnumberofmemoryblockstorecordhistogramsin(512).Again,press-
ingthespinbuttonswillapplychangesinthecorrespondingeditboxesimmediately;enteringvaluesmanually
requirepressing<Enter>ortheApplybutton.
TheIRFcanbemeasuredautomaticallyaspartofthescan,ataseparatelychosenwavelength.Todoso,
checktheboxentitledIRF atandenterthewavelengthatwhichtomeasuretheIRFinthecorrespondingedit
box.ThemonochromatorwillgototheIRFpositionatthebeginningoftheTRESmeasurement.AftertheIRF
wasmeasured,theactualTRESscanwillbeperformed.TheIRFwillalwaysberecordedinmemoryblock0,
whilethewavelengthscanalwaysstartsinblock1.NotethatstartingaTRESmeasurementwillclearandsub-
sequentlyoverwriteallcurvesinmemorythatmayhavebeenrecordedpreviously.Ifyouwishtomeasurethe
IRFyourself,youmustdothisAFTERtheTRESmeasurement.Ifthecurvesinmemorywereloadedfromafile,
theactualfileondiskwillnotbeoverwrittenuntilyousavetothefile(Savemenuorcorrespondingtoolbarbut-
ton).UseSaveAsandselectanewfilenameifyouwanttopreservetheoldfile.NotethataTRESfilewillre-
mainaTRESfileforitslifetime.Thismeansthataftercreatingorre-loadingsuchafileyoucandoonlytwo
things:performanewTRESmeasurement(effectivelyoverwritingtheoldfilecontents)ormeasureanIRFinto
curve0(overwritingonlycurve0).Ifyouwanttoperformanyothermeasurementyouwillneedtocreateanew
fileorloadanon-TRESfile.
TheTRESmeasurementisselectedintheMultiHarpcontrolpanel.Youneedtousetheselectionboxforthe
measurementmodetoselectTRESmode(insteadofOSCorINT).
Page55