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TA Instruments Nano ITC 5300 - User Manual

TA Instruments Nano ITC 5300
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Revision F Issued February 2011
Getting Started Guide for
Models 5300, 5301, 5302
Nano Isothermal
Titration Calorimeter
(Nano ITC)

Table of Contents

Questions and Answers

  • A
    Andrew HarrisAug 9, 2025
    Why is stirring speed important for TA Instruments Nano ITC 5300 Measuring Instruments?
    • H
      hollandjohnAug 9, 2025
      Stirring speed is important because, generally, a stirring speed of 250 to 400 rpm is appropriate for instruments made with gold sample and reference cells. In Hastelloy cell instruments, the best results are often obtained with stirring speeds of 150–200 rpm. Higher speeds generally result in higher noise levels in the baseline. Stirring too slowly may result in broad titration peaks that require longer intervals between injections.
  • D
    Deanna RamosAug 18, 2025
    How to minimize blank corrections in TA Instruments Nano ITC 5300 Measuring Instruments?
    • J
      Janice ChangAug 18, 2025
      To minimize blank corrections: * The ligand (titrant) solution should always be made up in the same dialysis buffer used for the protein (titrand). * If the ligand is also a protein, it should be dialyzed in the same buffer. * Less concentrated solutions also have lower dilution heats and should be used when possible.
  • A
    Ashlee WilliamsAug 28, 2025
    How to operate TA Instruments Nano ITC 5300 at non-ambient temperatures?
    • A
      Antonio HernandezAug 28, 2025
      When operating at non-ambient temperatures, it is important to allow adequate time between injections for the titrant to equilibrate to the calorimeter temperature before injection. Thorough degassing of the titrant is especially important when operating above the ambient temperature.

Summary

Notes, Cautions, and Warnings

Low Voltage Directive and Safety

Covers safety compliance and warnings for electrical safety.

Lifting the Instrument

Warnings about moving the instrument.

System Components

Instrument Specifications

Installing the Nano ITC

Instrument Installation Steps

Procedures for setup and recommendations.

Choosing a Location for the Nano ITC

Guidelines for ideal placement.

Power Requirements and Connection

Details power source, surge protection, and connection.

Setting Up the Nano ITC

Basic setup steps.

Connecting Cables and Cords

How to connect power and USB cables.

Starting the Nano ITC

Steps to power on and launch software.

Use, Maintenance, & Diagnostics

Calibrating Chemicals

Discusses calibration methods and examples.

Experiment Parameters for Calibration

Lists parameters for calibration.

Calibration Factor Input Location

Shows where to input calibration factor in software.

Preparing and Degassing Solutions

Detailed steps for preparing samples and buffers.

Degassing Solution Procedures

Preparing Sample and Reference Cells

Loading the Buret

Installing the Buret Assembly

Starting the Experiment

Steps to begin a titration experiment.

Cleaning the Nano ITC

Procedures for cleaning the instrument and its components.

Soapy Water Flush Procedure

Final Water Flush Procedure

Analyzing the Data

Nano ITC Software Functions

Troubleshooting the Nano ITC

Common issues and solutions.

Minimizing Blank Corrections

Optimal Stirring Speeds

Stable Instrument Operation

Typical System Variable Values

Buret Position Functions

TA Instruments Nano ITC 5300 Specifications

General IconGeneral
BrandTA Instruments
ModelNano ITC 5300
CategoryMeasuring Instruments
LanguageEnglish

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