Thermo Scientific Evolution 600 - Page 53

• Highlight Standards and press Enter to open the Standards Entry
page. Enter the concentration of each standard. Press the Accept
function key when all standards have been entered.
• There may be 1, 2 or 3 replicates per standard.
• Normally, Measure Standards is set to Yes. When set to No,
absorbance values may be entered manually.
• The minimum number of standards required will depend on the
curve fit.
Linear: 2
Linear through 0: 1
Quadratic: 3
Quadratic through 0: 2
Normally, a calibration contains more than the minimum number
of standards.
• Timers and UVcalc equations can be used, if required.
3. Press the Load Method function key to load a previously saved
method from the instrument Library or from disk.
4. Clear the beams or insert suitable blank(s) and close the lid of the
sample compartment.
5. Press Zero to zero the instrument.
6. Press the Calibrate function key.
Follow the on screen instructions, which will depend on whether a cell
programmer is in use and on the number of standards and replicates.
Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 49