4. Repeat these steps until you have specified the entire base sequence.
The displayed number of bases, %GC content, DNA Molecular
Weight, DNA absorptivity (extinction coefficient), and factor will be
updated as each new base is added to the sequence.
5. Press the T
m
Calculation function key to view the melting point
calculator.
66 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific
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