Waters ACQUITY Arc System - Page 78

Table B–5: Mobile phase absorbance measured against air or water (continued)
Absorbance (AU) at specified wavelength (nm)
200 205 210 215 220 230 240 250 260 280
Triethylamine, 1% 2.33 2.42 2.50 2.45 2.37 1.96 0.50 0.12 0.04 <0.01
Buffers and salts
Ammonium acetate, 10 mM 1.88 0.94 0.53 0.29 0.15 0.02 <0.01 — — —
Ammonium bicarbonate, 10 mM 0.41 0.10 0.01 <0.01 — — — — — —
Ethylenedinitrilotetraacetic acid disodium salt
(disodium EDTA), 1 mM
0.11 0.07 0.06 0.04 0.03 0.03 0.02 0.02 0.02 0.02
4-(2-hydroxyethy l)-1-
piperazineethanesulfonic acid (HEPES), 10
mM, pH 7.6
2.45 2.50 2.37 2.08 1.50 0.29 0.03 <0.01 — —
Morpholinoethanesulfonic acid (MES), 10 mM,
pH 6.0
2.42 2.38 1.89 0.90 0.45 0.06 <0.01 — — —
Potassium phosphate, monobasic (KH
2
PO
4
),
10 mM
0.03 <0.01 — — — — — — — —
Potassium phosphate, dibasic, (K
2
HPO
4
), 10
mM
0.53 0.16 0.05 0.01 <0.01 — — — — —
Sodium acetate, 10 mM 1.85 0.96 0.52 0.30 0.15 0.03 <0.01 — — —
Sodium chloride, 1 M 2.00 1.67 0.40 0.10 <0.01 — — — — —
Sodium citrate, 10 mM 2.48 2.84 2.31 2.02 1.49 0.54 0.12 0.03 0.02 0.01
Sodium formate, 10 mM 1.00 0.73 0.53 0.33 0.20 0.03 <0.01 — — —
Sodium phosphate, 100 mM, pH 6.8 1.99 0.75 0.19 0.06 0.02 0.01 0.01 0.01 0.01 <0.01
Tris HCl, 20 mM, pH 7.0 1.40 0.77 0.28 0.10 0.04 <0.01 — — — —
Tris HCl, 20 mM, pH 8.0 1.80 1.90 1.11 0.43 0.13 <0.01 — — — —
June 12, 2015, 715004747 Rev. A
Page 78