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number of living cells in a sample. For detailed information, refer to the application note
Apoptosis Detection by Annexin V and Active Caspase 3 with the Agilent 2100
Bioanalyzer.
Apoptotic cells
In apoptotic cells, phosphatidylserine is no longer confined to the inner leaflet of the
plasma membrane bilayer. Phosphatidylserine becomes accessible on the outer surface
of the cell membrane and can be bound with high affinity by the protein annexin V, which
can be labeled with biotin or dyes such as Cy5.
Gating direction
The gating direction is from blue fluorescence (living cells) to red fluorescence (annexin).
Histogram evaluation
The two histograms displaying the results of the assay are related to calcein (blue
fluorescence) and annexin V (red fluorescence). High fluorescence values in the blue
histogram indicate living cells, low values correspond to dead cells. See the following
image as an example.
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