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How to Create a Custom Assay
To create a custom assay:
1 Switch to the Assay context.
2 From the Assays menu, select an assay.
– OR –
Select File > Open and open an assay (.xsy) file.
The file appears in the Tree View Panel.
NOTE
If you want to create a new flow cytometric assay with free gating direction or with
more than one marker or region, open and modify the assay “Generic.xsy”.
3 Switch to the Assay Properties Tab to modify the assay setpoints if required.
4 Switch to the Chip Summary Tab to enter chip, sample, and study information.
5 For flow cytometric assays, define markers and regions on the Histogram Tab
(Single/Grid View) and Dot Plot Tab (Single/Grid View).
6 For electrophoretic assays, define flagging rules on the Result Flagging Tab.
7 Select File > Save As to open the Save As dialog box.
8 Under Save as type, select (.xsy), and enter a name and location for the new assay.
9 Click Save to create the new assay.
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