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5 Optimizing Performance
Optimization for Lowest Carry-over
Using an Injector Program
The process is based on a program that switches the bypass grove of the
injection valve into the flow line for cleaning. This switching event is
performed at the end of the equilibration time to ensure that the bypass grove
is filled with the start concentration of the mobile phase. Otherwise the
separation could be influenced, especially if microbore columns are used.
For example:
Outside wash of needle in vial 7 before injection
Injector program:
Draw x.x (y) µl from sample
NEEDLE WASH vial 7
Inject
Wait (equilibration time - see text above)
Valve bypass
Wait 0.2 min
Valve mainpass
Valve bypass
Valve mainpass
NOTE
Overlapped injection together with additional injection valve switching is not possible.
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