Page 22 of 59 BACTcontrol – User Manual – V0.4.4 microLAN b.v.
5.8 REAGENTS
The Buffer and reagents that can be used in the BACTcontrol:
Figure 11 Reagents overview
1. Cleaning solution concentrate: Contains sodium hypochlorite solution (<0.05% Cl
active).
2. Buffer reagent: Contains Tritron-X-100 and MOPSO (β-Hydroxy-4-
morpholinepropanesulfonic acid) which is a common buffering agent in biology and
biochemistry which is used to keep the pH value stable during a certain period of time.
3. E.coli reagent: Contains the fluorogenic substrate 4-Methylumbelliferyl β-D-glucuronide
(MUG) to which ß- glucuronidase enzymes present in E.coli bacteria will attach and will
hydrolyse.
4. Enterococci reagent: Contains the fluorogenic substrate 4-Methylumbelliferyl ß-D-
glucopyranoside (MUD) to which ß- glucosidase enzymes present in enterococci
bacteria will attach and will hydrolyse.
5. Coliform reagent: Contains the fluorogenic substrate 4-Methylumbelliferyl ß-D-
galactopyranoside (MUGal) to which ß- galactosidase enzymes present in coliform
bacteria will attach to and will hydrolyse.
6. Total activity reagent: Contains the fluorogenic substrate 4-methylumbelliferyl
phosphate (MUp) to which alkaline phosphatase enzymes present in all bacteria will
attach to and will hydrolyze. Since this reagent contains buffer, no extra buffer reagent
will be added when total activity measurement is started. The determination of total
activity is an optional measurement. Before starting this measurement, the reagent of
total activity should be placed in the BACTcontrol, replacing the E.coli, enterococci or
coliform reagents.
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