microLAN b.v. BACTcontrol – User Manual – V0.4.4 Page 23 of 59
5.8.1 EXPLANATION SUBSTRATE
The substrate contained in all three solutions mentioned above is Methylumbelliferone
(MU) in combination with another enzyme specific compound. MU is a fluorescent
indicator; it is colourless at pH 7.0 and exhibits a blue fluorescence at pH 7.5. The
fluorescence intensity of MU is pH dependent and increases to a maximum at pH 10 .
The fluorescence at pH 10.3 is approximately 100 times as intense as at pH 7. It is
therefore extremely important to add a buffer before each measurement or calibration,
so the fluorescence intensity remains stable (Sigma-Aldrich, n.d.)
MU Methylumbelliferone
MUG 4-Methylumbelliferyl β-D-glucuronide
MUD 4-Methylumbelliferyl ß-D-glucopyranoside
MUGal 4-Methylumbelliferyl ß-D-galactopyranoside
MUp 4-Methylumbelliferyl phosphate
rfu relative fluorescence units
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