BD FACSCelesta Flow Cytometer User’s Guide108
Dichroic mirrors Dichroic filters that are used to direct different color light signals
to different detectors are called dichroic mirrors.
Although some of the properties of LP and SP filters are similar to
dichroic mirrors (for example, allowing a specific wavelength
range to pass), filters and mirrors cannot be used interchangeably,
especially if used as dichroic mirrors. A dichroic mirror must have
a surface coating that reflects certain wavelengths, but many LP or
SP filters are absorbance filters that do not have any specific
reflective characteristics. Also, optical filters and dichroic mirrors
are rated at a specific angle of incidence. When used in front of the
fluorescence detectors, they are perpendicular to the incident light,
and when used as a dichroic mirror, they are placed at an angle
relative to the light source. Their optical properties are therefore
designed for that angle of incidence.
Wavelength (nm)
% Transmission
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