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BD FACSCelesta Flow Cytometer - Page 113

BD FACSCelesta Flow Cytometer
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Chapter 7: Technical overview 113
3. As the particle leaves the beam, the pulse trails off below the
threshold.
Pulse
measurements
The pulse processors measure pulses by three characteristics:
height, area, and width.
Height. The maximum digitized intensity measured for the
pulse.
Area. The integration of all the digitized samples over time,
where time is the window gate plus 1/2 the window extension
added before the initial threshold, plus the other half of the
window extension value added after the pulse drops below the
threshold. The window gate extends until the pulse is 75% of
the initial threshold.
Width. The current width is measured by the electronics.
Signal intensity
Time

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