BD FACSCelesta Flow Cytometer User’s Guide114
Digital electronics BD FACSCelesta electronics digitize the signal intensity produced
by a detector. The digitized data is stored in memory and further
processed by the electronics to calculate:
• Pulse height and area
• Compensation
• Parameter ratios
These results are transferred to your workstation computer for
further processing by BD
FACSDiva software. For more
information about digital theory, see Digital Theory in the
BD
FACSDiva Software Reference Manual.
Threshold The threshold is the level at which the system starts to measure
signal pulses. A threshold is defined for a specific detector signal.
The system continuously samples the digitized signal data and
calculates pulse area and height for all channels based on the time
interval during which the threshold is exceeded.
Thresholds can also be set for more than one parameter, and pulse
measures are based on either of the following:
• Intervals during which ALL signals exceed their threshold
value (AND threshold in the software)
• Intervals during which ANY signal exceeds its threshold value
(OR threshold in the software)
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