Chapter 3: Cytometer setup 37
3. Direct the vent line into a beaker and press the small button at
the end of the vent fitting against the side of the beaker until a
steady stream of fluid empties from the filter.
4. Tilt the filter and verify that no trapped air remains in the
filter.
5. Repeat steps 3 and 4 until no air is observed in the filter.
6. Check the sheath line for air bubbles.
7. Open the roller clamp at the fluidics interconnect (if necessary)
to bleed off any air in the line. Collect any excess fluid in a
waste container.
Note: The roller clamp can be found close to the fluidics ports
of the cytometer.
8. Close the roller clamp.
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