Chapter 4: Maintenance 51
6. Remove the DI water tube from the SIP.
7. Prime the instrument twice:
a. Press the PRIME button on the fluidics control panel.
b. When the STANDBY button light is amber, press the
PRIME button again.
8. Install a tube with 3 mL of BD FACSClean solution on the SIP
and put the tube support arm underneath the tube.
See Maintenance overview (page 46) for other recommended
cleaning solutions.
9. Press RUN and HIGH on the cytometer fluid control panel.
Ensure that the sample fine adjust is set to 250. Run for 30
minutes.
10. Press the STANDBY fluid control button and depressurize the
sheath container by lifting the vent valve.
11. Empty the waste tank to avoid the mixing of Bleach and BD
Detergent Solution.
12. Repeat steps 2 through 11 with 1.5% BD Detergent Solution
Concentrate.
13. Repeat steps 2 through 11 with DI water.
14. Replace the sheath filter and refill the sheath container with
sheath fluid.
15. Install a tube with less than 1mL DI water onto the SIP.
Caution: Biohazard! Never mix BD Detergent Solution
and bleach because they can create dangerous fumes.
To Next Page
Loading...
