Chapter 4: Maintenance 55
Removing the old
filter
To remove the old filter:
1. Place the cytometer in standby mode.
2. Remove the sheath filter assembly by pressing the
quick-disconnect on both sides of the filter assembly.
3. Over a sink or beaker:
• Remove the vent line from the filter and set it aside.
• Remove the filter base and set it aside.
4. Discard the used filter assembly in an appropriate receptacle.
Attaching the new
filter
To attach the new filter:
1. Connect the vent line to the new filter assembly.
Twist to attach.
2. Wrap Teflon® tape around the filter threads, then connect the
filter to the filter base.
3. Connect the sheath line to the filter assembly by squeezing the
quick-disconnect.
4. Attach the cytometer fluid line to the filter assembly via the
quick-disconnect.
5. Direct the vent line into a beaker and press the small button at
the end of the vent fitting against the side of the beaker until a
steady stream of fluid empties from the filter.
6. Tilt the filter and verify that no trapped air remains in the
filter.
7. Repeat steps 5 and 6 as necessary to remove all trapped air.