BD FACSCelesta Flow Cytometer - Page 78

BD FACSCelesta Flow Cytometer User’s Guide78
3. In the Cytometer window, optimize the PMT voltages for the
application.
• Optimize the FSC and SSC voltages to place the population
of interest on scale.
• Optimize the FSC threshold value to eliminate debris
without interfering with the population of interest.
• If needed, increase the fluorescence PMT voltages to place
the negative population appropriately for your sample
type.
Note: Do not decrease the fluorescence PMT voltages. Doing
so can make it difficult to resolve dim populations from the
negative population.
4. Unload the unstained control tube from the cytometer.
5. Load the multicolor sample onto the cytometer or load single-
color control tubes and verify each fluorochrome signal
separately.