100-214-239 Rev. A A-11
Appendix A
Digital Sonifier
Phosphatidate phosphohydrolase:
the most potent inhibitors for this enzyme were
obtained by making five dispersions with the Sonifier Cell Disr uptor.
Phospholipid micelles:
produced stable preparations for an indefinite period.
Plant cells:
30% packed plant cells (W/V) and distilled water (depending on type) can be com-
pletely disrupted in 1-15 minutes.
Plant tissue:
1 gm dried tissue suspended in alcohol disintegrates in about 5 minutes.
Platelets:
complete disruption according to size from 20 seconds to 4 minutes.
Pneumococci:
preserved in formalin for several years; completely disrupts in 6 minutes.
Polio virus:
excellent disruption of this most difficult virus.
Powders:
broken d own to a small, relatively uniform particle size.
PPLO:
complete disruption in 2 minutes.
Propionobacteria:
releases sulfhydro groups intact; 70 ml of 20% suspension processed for
10 minutes.
Propionibacterium shermanii:
2 minutes for extraction of citrate synthose .
Proteus:
excellent disruption.
Pseudomonas aeruginosa:
rapid, complete disintegration.
Pseudomonas fluorescens:
2 gm wet wt in 10 ml completely disrupts in 1 minute.
Pulmonary cytodiagnosis:
the mucous in sputum can be evenly dispersed, giving a quick
representative sample of cells for cytologic examination. Cells are liberated from the mucous
of sputum that had been immersed in 50% alcohol or a fixative.
Ragweed pollen:
15 ml dispersion completely disintegrates in 11 minutes.
Rat bone:
1/2 gm disintegrates in 4 minutes.
Rat liver:
complete disruption in 3 minutes.
Rat liver mitochondria:
ultrasonic treatment has been used extensively for the varied research
performed on this material. Disr uption occurs in seconds.
Rat skin:
1 gm completely disintegrates in 4 minutes.
Red and white blood cells:
ultrasonic treatment breaks particle size to 100 Angstroms. Com-
plete disruption in 1 minute. 25 gms/100 ml, saline or plasma, sample treated 15
seconds, 35% disruption. Adenosine triphosphate was shown to be membrane-bound by this
method.
Reovirus:
dissociates cell-bound and aggregated virus. Maximum titer with 4 ml of virus was
achieved in 2 minutes.
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