Gemini Series Electroporator User’s Manual
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45
Experiment Troubleshooting
Arcing
Verify electrical component functionality. Verify properties of cell
sample (do cells need to be washed? Is the buffer appropriate for
application?). Verify properties of transfectant molecule (Is the
DNA well purified?) Try reducing the voltage or increase sample
volume until arcing is no longer a problem.
Low (or no) Transfection Efficiency, or
Incorporation
(Verify physical, biological, chemical parameters) Verify delivery
of the pulse and pulse parameters. Is the voltage correct? Chamber
gap? Pulse length or appropriate instrument settings? Number of
pulses? If so, follow Optimization Guidelines outlined.
Low Viability
Verify physical, biological, chemical parameters. Is the voltage,
chamber gap, pulse length (time constant), pulse number and
other instrument settings correct? If so, reduce voltage, pulse
length, or number of pulses and re-optimize protocol to improve
viability as outlined .
Voltage Drop
A drop in output voltage accompanies pulse delivery into highly
conductive samples (for example, PBS). Thus the displayed voltage
may in these situations be less than that expected, given 5% full
scale accuracy and the monitoring accuracy of 5%.
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