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BTX Gemini X2 User Manual

BTX Gemini X2
48 pages
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Gemini Series
Twin Waveform
Electroporation Systems
User’s Manual
www.btxonline.com
5507-002 REV 1.0

Table of Contents

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BTX Gemini X2 Specifications

General IconGeneral
BrandBTX
ModelGemini X2
CategoryMeasuring Instruments
LanguageEnglish

Summary

Safety Information

High Voltage Risk

Details the risks associated with high voltage power supplies and precautions.

Product Overview

Gemini X2 vs Gemini SC Comparison

Highlights the key differences and intended uses of the Gemini X2 and Gemini SC systems.

General Optimization Guide for Electroporation

Wave Forms

Explains the use of square wave and exponential decay waveforms for electroporation.

Field Strength

Details factors influencing field strength and provides ranges for different cell types.

Applications

Mammalian Cell Transfection

Describes the use of electroporation for genetically modifying mammalian cells.

Unpacking the System

Installation

Guidance on setting up the generator, connecting components, and initial power-up.

Software Setup

Preset Protocols

Performing Experiments

Creating New Protocols

General steps for creating new custom electroporation protocols.

Creating an Exponential Decay Wave Protocol

Detailed instructions for configuring exponential decay pulse parameters.

Creating a Square Wave Protocol

Detailed instructions for configuring square wave pulse parameters.

Using Specialty Protocols

Using an HT Plate Handler, Gemini X2 only

Instructions for connecting and operating the HT Plate Handler accessory.

Using Specialty Electrodes, Gemini X2 Only

Guide to connecting and using various specialized electrodes.

Managing Protocols

Protocol Manager Software

Remote Control Software

Maintenance

Upgrading Gemini Series Software

Device Updater Program

Instructions for updating the generator's firmware using the Device Updater.

Error Messages & Troubleshooting

Troubleshooting

Experiment Troubleshooting

Arcing

Troubleshooting steps to resolve electrical arcing issues during experiments.

Low (or no) Transfection Efficiency, or Incorporation

Diagnosing and resolving issues with poor transfection or molecule incorporation.

Warranty Information

Warranty

Details the two-year warranty period, terms, and conditions for repair or replacement.