for 20 min at 121℃（1.0 kg/cm
2
）. Too high temperature would result in deformation of centrifuge tube.
When autoclave is used, take the following steps:
（1） Place the centrifuge tube upright with opening facing upward. If the centrifuge tube is placed
in an inclined or horizontal manner, it will deform due to the effect of gravity.
（2） Remove the threaded cover and inner cover to prevent deformation or crack of the centrifuge
tube.
（3） Take the centrifuge tube only when the autoclave cools down to the room temperature.
11.2.4. Service life of centrifuge tube
The service life of plastic centrifuge tube depends upon the nature of sample, rotor speed and
centrifugation temperature. When the plastic centrifuge tube is used for centrifugation of conventional
neutral samples (pH5-pH9), its estimated service life at the maximum rotation speed is as follows:
High-quality centrifuge tube (PA, PC, PP): 30-50 times.
Conventional centrifuge tube: about 10 times (frequency of use may be increased in case of low-speed
application use)
The service life of centrifuge tube is also related to the cleaning and sterilization conditions.
Note: Never use any centrifuge tube with cracks.
12. RCF calculation
Relative centrifugal force (RCF）can be calculated using the following formula:
RCF=1.118×r×n
2
×10
-5
r-rotation radius, unit: cm; n-rotation speed, unit- rpm
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