Theoretical Principles
User Manual Hemo Control
Figure 25 – Principle of photometric measurement
P
0
: 100 % - light intensity,
P: remaining light intensity,
b: distance through the solution
Equation 1 – Absorbance A
Equation 2 - Lambert-Beers Law
: molar extinction coefficient
b: distance through solution in the microcuvette
c: concentration of solution
Using Lambert-Beers Law, (refer to Equation 2) the concentration of the
hemoglobin in the microcuvette can be determined. To this end the
equations are solved for the concentration c.
where
Equation 3 - Calculation of concentration
k: proportionality factor for measurement method correction
(taking into account design conditions)