6.3 Start a method run
Prerequisites
Make sure that the system is correctly prepared. Confirm the following:
•
The sample inlet is primed or the sample loop is loaded.
•
The column is properly connected.
•
The pressure alarm has an appropriate limit set.
•
That there is no air in the system.
•
The buffer inlet tubing(s) is immersed in correct buffer vessels.
•
All waste tubing is immersed in appropriate waste vessels that have sufficient empty
volume.
•
No tubing is twisted and the flow path is free from leakage.
•
If a Fraction collector is being used, that the correct tubes are used and loaded.
Start a method run
Follow the steps below to start the method run.
ActionStep
Open the System Control module and click the button Open Method Navi-
gator.
1
Select the method to run, and click the Run button.2
Step through the displayed pages in the Start Protocol, add requested input
and make appropriate changes if necessary. Click Next.
3
Click Start on the last page of the Start Protocol.4
84 ÄKTA go Operating Instructions 29360951 AC
6 Run a method
6.3 Start a method run
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