•
To apply all sample feed onto the column, use a short piece of tubing between the
external air sensor and the inlet valve. Remember to have the air trap in-line while
applying sample using short tubing and high flow rates, because air might pass be-
yond the inlet valve.
•
To prevent air entering the system flow path, place the external air sensor close to
the liquid container. When running high flow rates, use a long (>70 cm) piece of
tubing between the external air sensor and the inlet valve to make sure no air
reaches the inlet valve.
In a predefined method, in the Sample application phase, the External air sensor 1 can
be used to facilitate sample application, see Section 4.1 Sample application, on page 60.
Air sensor sensitivity
The air sensor has two sensitivity levels, High that detects down to approximately 30 μL
bubbles, and Normal that detects down to approximately 1 mL bubbles. The level Normal
is default for external air sensors.
To change the sensitivity, select System:Settings in the System Control module to open
the System Settings dialog box. Select the external air sensor in the Air sensor list, and
set the sensitivity as required, see illustration below.
Air sensor alarm
The air sensor alarms are disabled by default. If an alarm is enabled, detected air pauses
the pumps.
To enable the alarm, select System:Settings in the System Control module to open the
System Settings dialog box. Select external air sensor in the Air sensor list, select Enabled
and click OK, see illustration below.
ÄKTA pilot 600 User Manual 29274559 AA 31
3 Description of modules
3.1 External air sensors (optional)
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