As variation in temperature influences conductivity readings, the conductivity flow cell
is fitted with a temperature sensor that measures the temperature of the eluent. A
temperature compensation factor is used to report the conductivity in relation to a set
reference temperature. The measured temperature is also used for pH electrode com-
pensation.
Pre-column conductivity monitor
If a conductivity monitor is installed in the flow path before the column valve, it can be
used
•
to follow conductivity during washing of pumps and valves before the column,
•
to monitor that the sample feed has suitable conductivity for the chromatography
run,
•
to monitor that column equilibration is completed by comparing conductivity levels
before and after the column,
•
to monitor that all sample has been wash out from the air trap by comparing con-
ductivity of the sample and buffer used to finalize sample application (if using the
air trap during sample application).
Conductivity monitor
(post-column)
The conductivity monitor after the column can be used
•
to measure the conductivity of salt gradients during gradient elution,
•
to monitor washing of the system, with the column(s) by-passed,
•
to monitor that column equilibration is completed by comparing conductivity levels
with column in-line and by-passed,
•
to follow peak positions relative to conductivity.
52 ÄKTA pilot 600 User Manual 29274559 AA
3 Description of modules
3.9 Conductivity monitor
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