DescriptionSetting
Shows the column valve position used by the Superloop.
This setting can only be changed by changing the Column
position in Method Settings.
Loop position
The specified volume will be pumped through the Superloop
on to the column. If it is important that all of the sample is
applied to the column, enter a volume approximately 10 mL
greater than the volume of sample in the Superloop.
Empty loop with
This option controls the source of the buffer pumped into
the Superloop eluent chamber during sample application.
Use the same inlets as
Method Settings
Select this option to fill the system flow path with the buffer
before applying the sample to the column.
Fill the system with
selected buffer
Settings specified here determine how flowthrough during
sample application will be collected. See Section 4.2 Frac-
tionation, on page 71 for details of fractionation settings.
Fractionation during sample application is generally rele-
vant only for adsorption chromatography.
Fractionation settings
70 ÄKTA pilot 600 User Manual 29274559 AA
4 Operation
4.1 Sample application
4.1.3 Apply sample from a Superloop
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