Use equilibrated solutions
Always use solutions equilibrated to the ambient temperature, for example, sample so-
lutions, buffers, and standard buffers for pH calibration. Temperature differences might
for example increase outgassing.
Higher viscosity
Generally, cold solutions have higher viscosity than warm solutions. The flow rate might
need to be lowered to keep an acceptable back pressure.
88 ÄKTA pilot 600 User Manual 29274559 AA
4 Operation
4.6 Performing runs in a cold environment
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