GE Analytical Instruments ©2006 8-1 DLM 14291 Rev. A
8. OFF-LINE EXHALED NITRIC OXIDE (BAG SAMPLING)
The concentration of nitric oxide in exhaled breath can also be measured by
collecting expired air in a Mylar balloon or Tedlar gas sampling bag and analyzing
the contents of bag. Sievers offers two systems for the off-line collection and
analysis exhaled NO.
The Vital Capacity Bag Collection and Sampling Kit (BSK 01410) meets the ATS
“Recommendations for Standardized Procedures for the Online and Offline
Measurement of Exhaled Nitric Oxide in Adults and Children-1999” (Am J Respir
Crit Care Med Vol 160. pp 2104–2117, 1999). Subjects exhale their vital capacity at
a flow rate of 0.35 L/s (±10%) at Body Temperature and Pressure, Saturated with
water vapor (BTPS) into a 12L Mylar bag.
The Deadspace Discard Bag Collection and Sampling Kit (BSK 01400) permits
collection of exhaled breath at the same flow rate (0.05 L/s) employed for on-line
measurements. A divert value in the filler permits discarding of the first portion of
the exhalation (physiological deadspace) and then a small sample (~300 mL) of
exhaled breath is collected in a 1.5 L Mylar bag equipped with a self-sealing valve.
Assembly of Vital Capacity Bag Collection Kit
The bag collection kit must be assembled before use (refer to Figure 8-1). To
assemble the kit:
• Remove all packages from the shipping container. Remove the Body of the
Sampler (the check valves and adapters are already installed).
• Screw the pressure gauge into the bag filler body (Teflon tape is not required).
• Inspect the pressure gauge. If the needle does not read 0 cm H
2
O, unscrew the
clear plastic cover of the gauge and adjust the screw at the base of the gauge
with a screwdriver until the needle points to 0 cm H
2
O. Replace the clear
plastic cover of the gauge.
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