GE Analytical Instruments ©2006 8-7 DLM 14291 Rev. A
Sample collection using the Vital Capacity Bag kit consists of three steps:
• Connecting a pre-cleaned bag to the filler.
• Instructing the subject on how to perform the maneuver and collecting the
samples
• Disconnecting the bag from the filler and sealing the bag.
Connecting the bag to the filler
To prepare the filler for sample collection:
• Stand the filler on the inspiratory filter.
• Remove the cap from the bag and grasp the cap, the top of the bag and
internal tube with one hand while holding the bag filler with the other hand.
• Twist the bag slightly counterclockwise, then insert the Luer fitting on the bag
into the Luer fitting on the filler and rotate fully clockwise until the o-ring
seals against the base of the bag filler.
• Rotate the bag so that there are no folds or restrictions at the end of the tube.
• Open a new viral and bacterial filter and connect the filter to the tapered end
on the top of the filler.
• Connect a sterile mouthpiece to the filter.
Instructing the Subject and Collecting the Samples
To perform the maneuver:
• Have the subject comfortably seated and holding the bag filler level.
• Have the subject place the mouthpiece in their mouth.
• Instruct the subject to inhale orally
through the inspiratory filter to total lung
capacity.
• Have the subject immediately exhale while watching the pressure gauge and
adjusting the force of exhalation to maintain a pressure of 13 cm H
2
O to give
an expiratory flow rate of 0.35 L/s.
* During the exhalation, the pressure should
* at 760 torr, for other pressures, calculate the required pressure from the equation:
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