GE Analytical Instruments ©2006 8-8 DLM 14291 Rev. A
always be greater than 10.5 cm H
2
O (0.315 L/s) but less than 15 cm H
2
O (0.385
L/s).
• The subject must continue exhaling to residual capacity while maintaining this
pressure.
If ambient NO levels are greater than 20-40 ppb, subjects should breath from a
source of low NO air or through the inspiratory filter for 15 s (or a minimum of two
tidal breaths) before the collection of the sample.
Make sure the o-ring on the bag is tightly sealed against the filler and that there
are no folds or restrictions in the bag.
WARNING:
IF THE FILLER IS NOT HELD LEVEL, THE BAG MAY FOLD AGAINST THE END OF
THE INTERNAL TUBE, RESTRICTING THE FLOW OF AIR INTO THE BAG.
Disconnecting the bag from the filler and sealing the bag.
After the subject has completed the maneuver:
• Stand the bag filler on the inspiratory filter.
• Grasp the Luer fitting and tube on the bag, rotate counterclockwise to
disconnect the bag from the filler.
• Seal the Mylar Bag with the cap.
Repeat the above procedures to connect another bag to the filler for replicate
measurements. Normally, subjects will fill three bags, and the average NO
concentrations in these three samples is used for the subject’s FE
NO, 0.35.
Subjects
should rest a few minutes between exhalations.
Flow = (760/P
Bar
(torr)) * 79.3 * P
exh
0.5851
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